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Physical and chemical pretreatments vary depending on the type of material and the depositional environment from which it came.
It is important to understand the nature of the material being dated as certain organic fractions are more appropriate than others, depending on the environment.
In many lacustrine environments, macrofossils are the preferred organic fraction used for dating.
Avoid porous (cancellous tissue) or soft bones as these are aslo likely to be low in collagen.
For δ13C and δ15N, we submit the extracted collagen to our partner lab, the .
The general theory is that humic acids percolate downward and make radiocarbon dates appear younger.
However, this is not always the case so it is important to test this method first before pursuing numerous analyses.